Short-term in vitro experiments (metabolism, effect on the microbial community, stability in upper and lower intestine)

SHIME-experiments: short- and long-term evaluation of the intetinal fate of specific compounds or formulations

Absorption modeling(eg. Dialysis, Ultrafiltration or Caco2-cell cultures)

In vivo validation using animal models

Strain characterization

Proof-of-concept validation in human trials

Identification of active compounds or precursors

Isolation of microorganisms for biotechnological applications

Development of production startegies

Formulation of the end product